human breast cancer tissue microarray slides  (Novus Biologicals)

Journal: PLoS ONE

Article Title: Evaluation of STAT3 Signaling in ALDH+ and ALDH+/CD44+/CD24− Subpopulations of Breast Cancer Cells

doi: 10.1371/journal.pone.0082821

Figure Lengend Snippet: ( A ) Representative flow cytometry analysis of ALDH enzymatic activity in SUM159 breast cancer cells was shown. ( B ) ALDH + and ALDH − subpopulations were separated from MDA-MB-231, SUM159, and SK-BR-3 breast cancer cells by flow cytometry. Phosphorylation of STAT3 (Y705), and ERK 1/2 (T202/Y204), was detected by Western blot. ( C ) Breast cancer tissue microarray slides were stained using immunohistochemistry (IHC). Representative examples of ALDH1 positive/P-STAT3 (Y705) positive (>95%, sample a) and ALDH1 negative/P-STAT3 (Y705 negative (<5%, sample b) tumors were shown. The spots for ALDH1 and P-STAT3 (Y705) were from the matched tissues section from the same patient. ( D ) Breast cancer tissue microarray slides were double-stained with P-STAT3 and ALDH1. Representative examples of the expression of STAT3 phosphorylation and ALDH1 was shown by immufluorence (IF) staining. ALDH1 high expression tumor cells (cytoplasm, green) also expressed P-STAT3 in nuclear (red).

Article Snippet: Human breast cancer tissue microarray slides, comprising 95 breast cancer cases, were obtained from the Biochain Institute, Inc. .

Techniques: Flow Cytometry, Activity Assay, Phospho-proteomics, Western Blot, Microarray, Staining, Immunohistochemistry, Expressing